save data for card 2 games (Animal crossing is one of them) is stocked and the end of the rom directly.
if you know how to use an hexadecimal editor, open your animal crossing rom with the editor and search for data written after all the rom partitions. Fill them with "FF" and you're done.
If you never use hexa edits, just trim your rom, and then untrim it. The save data will be wipped and ROM refilled with padding by doing so.
edit : not sure about this solution, but there is maybe a combo key that you have to press at game's launch to force it to erase your save. I know many Nintendo games can do this. dunno if it works with backups.