As in they're two separate ISOs and not one split into two files? If they are just separate ISOs, I can't think of any way to safely merge them and it work. Theoretically, with a lot of work you could build an eboot out of them, but that would be an insane amount of work for a minor convenience, and I wouldn't even know where you would start.
If they're just a split ISO, you should be able to run something like:
Code:
cat Part1.iso Part2.iso > Complete.iso
Well, using a linux distro with a terminal. Not sure about under Windows.