Nope, but 64k cluster size is recommended. Fixes things in certain games, like twice as fast load times in TPHD and fixes lag in Bayonetta 2. Use GUIFormat to set it.
After reformatting it works! thanks!
Note: I have used the command:
sudo newfs_msdos -b 65536 /dev/disk1s1
from Mac OSX El Capitan if anyone is interested. Make sure /dev/disk1s1 is your sd card!