reinstall kernel, if still doesn't work, maybe the card is defective
Please download the kernel here, unzip it can copy all files to root the memory card

if no bootloader icon is showing up then you either have a contact issue or a corrupted bootloader.
http://gbatemp.net/threads/solving-acekard-contact-issues.231412/
http://wiki.gbatemp.net/wiki/Updating_the_AK2i_Bootloader
the kernel (akaio) won't load until the card boots, so reinstalling akaio or updating it isn't going to solve your problem.
-another world