Ermm ... I think you're a little confused on what the Ak2i 1.4 update does
What the update does is rewrite the EEPROM memory of the actual card (note this is NOT the MicroSD memory card that put into it) so that it bypasses the DSi firmware update & fools the DSi that a genuine card has been inserted into Slot-1
What you are suggesting is somehow using the required update on a PC through a Gameboy software emulator, which has no way of allowing you to attach the Ak2i to the PC
The only device I've ever come across that allows you to attach a game card to a PC through a USB device is the NDS Adaptor - but this only allows you to backup the sav data of a commercial game
The other problem is that IIRC NO$GBA doesn't have any USB support built in
Moved to correct section..and just a little tip, even if it isn't against the rules, abuse of the emoticons can push away people, so you're likely to get less replies..
You might want to use one-two emoticons per reply