I know USB3.0 should support 2.0, but I think the problem might be the loader as my HDD works fine in neogamma but doesn't work in USBloaderGX.I think it depends on the loader.I'll check if my HDD is supportedUSB3.0 supports 2.0 interfaces, so it should probably be fine unless you have a 3TB+ partition, I think there were problems with that.