usally No. most games trim just fine. Roms tend to be "Bigger" than whats actually on the card. Dumps are kinda like..... an ISO and can end up having "Dummy" Space that fills the unused portion of the game cards Capacity.
if you want to be sure, test it in something like no$DS, (or did they go back "no$gba"?), DeSuMe, melonDS to see if it boots properly.