1 - Use a hex editor to look at the fourth byte in the ISO;
use "Open Partially" open the first 100 bytes or so, the 4th letter will be E/X or J or U, for pal, and ntsc.
2 - Use WUM, and open the ISO, it will tell you the region.
3 - Use Trucha signer to open the ISO. The 2nd partition will...