possibly. the only issue is that no model of AceKard has been updated to work with the 3DS firmware 4.4.0, so it would only work if the 3DS had a firmware lower than that. the AceKard team says that they're working on it, but it's been a while and people are having their doubts that they will release a patch.
and of course, only if it is a legitimate AceKard2i with a sticker on its back. the original models without the sticker from before the DSi was released in North America hasn't been updated in ages.