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If you're asking in the context of this thread, which is about a DSTWO MIPS processor plugin, the answer is a definite no
But recent Desmume versions can emulate filesystems for an R4 card. Go to Slot 1 emulation options and select your R4 filesystem folder.
Ah, makes sense, I wanted to test the emulator, but I don't have the DSTWO card, but I was just curious is all. Thanks for the info! Didn't know the emulator could load an "R4".