of course no. In fact,there have so many flashcart can directly compatibility 3ds v2.1.0_4 or higher version without any updating.Such as : dstwo,ak2i,r4i gold 3ds and so on . Maybe as you have known,now,the latest 3ds firmware is v3.0.0-6,if you want make your flashcart working on v3.0.0-6 as well.You can update your 3ds firmware.But note , never udpate your 3ds firmware before updating your card.